Dr. Frank Gordon Oppenheim, DDS

Frank Oppenheim, DMD, PhD Professor and Chair, Department of Periodontology and Oral Biology, Boston University School of Dental Medicine [email protected] Education Matura B, Gymnasium Aarau, Switzerland D. Med. Dent., University of Zurich, Switzerland DMD, Boston University CAGS in Periodontology, Boston University Ph.D. in Biochemistry, Boston University Research Goals The work of my laboratory focuses on the elucidation of structure and function of salivary proteins, which play important role in host defense mechanisms. Structural analysis, peptide biomimetics employing solid phase peptide synthesis and recombinant expression methods are being used in the identification of functional domains within proteins. Functional investigations comprise mechanisms related to anti-bacterial, anti-fungal and mineral homeostasis. Emphasis on these processes is aimed at understanding the basic physiology of hard and soft tissue protection in the oral environment. Proteins such as the histatins, the proline-rich proteins and statherin are examples of proteins unique to the oral cavity. For example, the discovery of the histatin protein family showed that these small basic proteins are rich in histidine, exhibit anti-microbial properties, contain specific functional domains, and are able to kill yeasts by entering cells and target mitochondria. The mechanisms of cell death is being studied. and it has been shown that the killing effect of histatins is related to the inhibition of the respiratory chain leading to the formation reactive oxygen radicals. Other studies in progress comprise the identification and characterization of salivary protein which show selective adsorption to hydroxyapaptite forming a unique protein film on the mineral surfaces of teeth. This protein film determines de- and re-mineralization processes and dictates the early events of microbial colonization.. These studies include biophysical techniques such as NMR and mass spectrometry. Approaches of proteomics are being used to characterize proteins and peptides formed on mineral surfaces in vivo. Recent Publications Gusman H, Travis J, Helmerhorst EJ, Potempa J, Troxler RF, Oppenheim FG. Salivary Histatan 5 is an inhibitor of both host and bacterial enzymes implicated in periodontal disease. Infect Immun 2001. 69:1402-1408. Grogan J, McKnight CJ, Troxler RF, Oppenheim FG. Zinc and copper bind to unique sites of histatin 5. FEBS Lett 2001. 491:76-80. Helmerhorst EJ, van’t Hof W, Breeuwer P, Veerman ECI, Abee T, Troxler RF, Nieuw Amerongen AV, Oppenheim FG. Characterization of histatin 5 with respect to amphipathicity, hydrophobicity, and effects on cell and mitochondrialmembrane integrity excludes a candidacidal mechanism of pore formation. J Biol Chem2001. 276:5643-5649. Gusman H, Grogan J, Kagan HM, Troxler RF, Oppenheim FG. Salivary histatin 5 is a potent competitive inhibitor of the cysteine proteinase clostripain. FEBS Lett 2001. 489:97-100. Gusman H, Lendenmann U, Grogan J, Troxler RF and Oppenheim FG. Is salivary histatin 5 a metallopeptide? Biochim Biophys Acta 2000. 1545:86-95. Yao Y, Lamkin MS and Oppenheim FG. Pellicle precursor protein crosslinking: characterization of an adduct between acidic proline-rich protein (PRP-1) and statherin generated by transglutaminase. J Dent Res 2000. 930-938. Gyurko C, Lendenmann U, Troxler RF, and Oppenheim FG. Candida albicans mutants deficient in respiration are resistant to the small cationic salivary antimibrobial peptide histatin 5.Antimicrobial Agents and Chemotherapy 2000. 44:348-354. Recent Grants Microbicidal Salivary Histidine-Rich Proteins, NIDCR-RO1 Anionic Salivary Proteins in Dental Integuments, NIDCR-RO1 Postdoctoral Training Program In Oral Biology, NIDCR-T32